- NCBI Gene Id:
- Protein Name:
- Ig gamma-1 chain C region, membrane-bound form
- Alias Symbols:
- IgG1, Igh-4, VH7183, Ighg1
- Replacement Item:
- This antibody may replace item sc-52003 from Santa Cruz Biotechnology.
- Description of Target:
- GOAT ANTI MOUSE IgG1:FITC
- Protein Size (# AA):
- IHC-AFF, FC, IF
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express Ighg1.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express Ighg1.
- The immunogen for anti-Ighg1 antibody: mouse IgG1 paraproteins
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-Ighg1 antibody - OASA06598
- Polyclonal IgG
- Application Info:
- Flow Cytometry: Neat - 1/10
Immunofluorescence: Neat - 1/10
- Preservative Stabilisers: 0.09% Sodium Azide (NaN3)
Antiserum Preparation: Antisera to mouse IgG1 were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
- Protocol Information:
- Citation: 1: Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH. Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles. Am J Pathol. 2006 Jul;169(1):200-8. PubMed PMID: 16816373; PubMed Central PMCID: PMC1698775.
Experiment Name: 1. Immunohistochemistry and Dual-Color Co-Immunofluorescence2. Soluble HCV E2 Binding and Blocking Assay
Experiment Background: 1. Immunofluorescent staining of mannose receptor and CD68 (green) was used to detect Kupffer cells, and DC-SIGN stained red. Colocalization of the two receptors is seen as yellow staining confirming that Kupffer cells express DC-SIGN in the liver2. To assess whether DC-SIGN and DC-SIGNR expressed in normal liver are able to interact with HCV E2, intact liver sections were incubated with truncated E2 from HC-J4 E2661 and bound antigen visualized with rat anti-E2 antibody 9/75, which recognizes the CD81-binding site on E2 and hence fails to interact with E2-CD81 complexes.
Experimental Steps: 1. Immunohistochemistry and Dual-Color Co-Immunofluorescence: The following primary antibodies were used: DC-SIGN (MAB161, IgG2b) and DC-SIGNR (MAB162, IgG2b) from R&D Systems, LYVE-1 (8C, IgG1; a gift from David Jackson, University of Oxford), CD68 (EBM11, IgG1; from Abcam, Cambridge, UK), and mannose receptor (MCA2155, IgG1; from Ltd., Oxford, UK). 5-um cryostat sections derived from normal liver were fixed in acetone for 10 minutes and stained using a standard alkaline phosphatase anti-alkaline phosphatase technique. Briefly, primary antibody was followed by rabbit anti-mouse monoclonal and mouse monoclonal alkaline phosphatase anti-alkaline phosphatase (Dako). The stain was developed with fast red and naphthol AS-MX phosphate substrate (Sigma-Aldrich). Sections for dual immunofluorescence were prewetted with staining buffer (phosphate-buffered saline containing 10% fetal calf serum and 0.1% sodium azide) for 10 minutes. Slides were incubated with primary antibodies diluted in staining buffer for 1 hour in a humidified chamber. Control sections were incubated with isotype-matched IgG2b or IgG1 (R&D Systems). Sections were stained with goat anti-mouse IgG2b Alexa Fluor (Molecular Probes, Eugene, OR) and goat anti-mouse IgG1 fluorescein isothiocyanate. Immunofluorescence was assessed using AxioVision software (Carl Zeiss MicroImaging, Inc., Jena, Germany).2. Soluble HCV E2 Binding and Blocking Assay: 293-T cells were transiently transfected with plasmids expressing HC-J4 E2661 or vector alone (control mock antigen) with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Tissue culture supernatants containing HC-J4 E2661 were harvested 48 hours after transfection, and the amount of HC-J4 E2 antigen was quantified by enzyme immunoassay. Normal liver sections were preincubated with isotypematched IgG2b (control block), DC-SIGN, DC-SIGNR (5 ug/ml; both from R&D Systems), or mannan (20 ug/ml; Sigma-Aldrich) for 1 hour, washed and then incubated with mock antigen or HC-J4 E2661 at a saturating concentration in phosphate-buffered saline/1% fetal bovine serum/0.05% sodium azide/1 mmol/L CaCl2 for 1 hour at room temperature, washed, and labeled with rat anti-E2 mAb 9/75, which recognizes the CD81-binding site on E2 and hence fails to interact with E2-CD81 complexes. Detection of binding on normal liver section was as described above using immunofluorescence with goat anti-rat fluorescein isothiocyanate. Immunofluorescence was assessed using AxioVision software (Carl Zeiss MicroImaging, Inc.).
Number Of Protocols: 2