Catalog No: OKDD00330 (Formerly GWB-KBBTL8)
Size:96 Wells
Price: $650.00
SKU
OKDD00330
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology IgG3 ELISA Kit (Human) (OKDD00330) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for IgG3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IgG3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IgG3 captured in well.
ELISA Kit Range0.156-10 ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IgG3 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IgG3 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-IgG3 Microplate96 Wells (12 x 8 Well Strips)
IgG3 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X IgG3 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of IgG3. No significant cross-reactivity or interference between IgG3 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolIgG3
Gene Full Nameimmunoglobulin heavy constant gamma 3 (G3m marker)
Alias SymbolsIgG3
NCBI Gene Id3502
Uniprot IDP01860
Protein Size (# AA)377
Molecular Weight41 kDa
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