IgG2a NEGATIVE CONTROL:RPE Negative/Isotype Control (OOSA10403)

Catalog No: OOSA10403 (Formerly GWB-E67EB5)

Size:100T

Product Info

• Predicted Species Reactivity: Negative Control
• Product Format: Lyophilized. Phosphate buffered saline
• Isotype: IgG2a
• Conjugation: R-PE
• Application: Flow cytometry
• Additional Information: Buffer solution: Phosphate buffered saline pH7.4
  Fusion Partner: Spleen cells from BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
  Immunogen: Activated rat T-helper cells.
  Label Protein Molar Ratio: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  Preservative: 0.09% - Sodium Azide
  1% - Bovine Serum Albumin
• Reconstitution and Storage: 2°C to 8°C
• Specificity: This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.
  This antibody may not be suitable for intracellular staining on some cell types.
  Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.
  This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.
  Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
  
• Predicted Homology Based on Immunogen Sequence: Negative Control
• Reference: 1. Avigdor, A. et al. (2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow. Blood. 103: 2981-2989.
  
• Immunogen: Activated rat T-helper cells.
• Purification: Protein G Purified

Target Info

• Description of Target: MOUSE IgG2a NEGATIVE CONTROL:RPE