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Catalog No: OKIA00104 (Formerly GWB-CF2E7B)
Size:1x96WELLPLATE
Price: $375.00
SKU
OKIA00104
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration90 Minutes
ELISA Kit PrincipleAviva Systems Biology IgG2A ELISA Kit (Mouse) (OKIA00104) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgG2A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgG2A - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG2A captured in the well.
ELISA Kit Range12.5 ng/ml - 400 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Mouse IgG2A Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Mouse IgG2A Calibrator1
Additional InformationIntended Use: The IgG2A test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG2A in biological samples of mice.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Mouse IgG2A antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-MOUSE IgG2A ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse IgG2A.
7. MOUSE IgG2A CALIBRATOR
One vial containing Mouse IgG2A calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.861 ng/ml
Predicted Homology Based on Immunogen SequenceMouse
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protein NameIgG2A
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