Catalog No: OKGD00001 (Formerly GWB-EKA001)
Size:96 Wells
Price: $439.00
SKU
OKGD00001
Availability: Domestic: within 1-2 days delivery | International: 1-2 days
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKGD00001
Product Info
Predicted Species ReactivityHuman
Product FormatLiquid
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~2 hours
ELISA Kit PrincipleAviva Systems Biology IGG FC ELISA Kit (human) (OKGD00001) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGG FC has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for IGG FC is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGG FC captured in well.
ELISA Kit Range0.3 - 250 ng/ml
ELISA Kit Component
ComponentQuantity
Anti-IGG FC Microplate96 Wells (12 x 8 Well strips)
IGG FC Lyophilized Standard2 vials
100X IGG FC HRP-Detection Anitbody1 x 10 uL
Assay Diluent1 x 30 mL
20X Wash Buffer1 x 30 mL
TMB Substrate1 x 12 mL
Stop Solution1 x 12 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Other Biological Fluids
Gene SymbolIGG FC
Gene Full NameImmunoglobulin G
NCBI Gene Id3500
Protein NameImmunoglobulin heavy constant gamma 1
Description of TargetIgG is the most abundant immunoglobulin in plasma, found at a concentration of 8 to 18 mg/ml. This immunoglobulin plays a very important role in the defense against infection for newborns because of its transfer through the placenta during pregnancy. It readily diffuses into the extravascular body spaces where it plays a major role in neutralizing bacterial toxins and in enhancing the phagocytosis of microorganisms. IgG binds to bacteria and these complexes adhere to phagocytic cells which have surface receptors specific for IgG.
Uniprot IDP01857
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