Catalog No: OKEH00512
Size:96T
Price: $275.00
SKU
OKEH00512
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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IgG ELISA Kit (Human) : 96 Wells (OKEH00512)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00512
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IgG ELISA Kit (Human) (OKEH00512) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human IgG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human IgG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human IgG captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Recovery<= 4.4%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-IgG Microplate96 Wells (12 x 8 Well strips)
IgG Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated IgG1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity29.2 pg/mL
SpecificityNatural and recombinant Human Immunoglobulin G
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIGG
Description of TargetIgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response.
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