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IgG Antibody (OARA05254)

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Description of Target:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Anti-Bovine IgG (H&L) antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Anti-Bovine IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Bovine IgG, whole molecule
Product Format:
Liquid 0.01 Sodium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 0.01% (w/v) sodium azide
Reconstitution and Storage:
Store vial at 4°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid.   Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
2.0 mg/ml (by UV absorbance at 280 nm)
Printable datasheet for OARA05254
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Bovine IgG and Bovine Serum.
Application Info:
Anti-Bovine IgG (H&L) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:20000~100000
IHC: 1:1000~5000
WB: 1:2000~10000

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