IGFBP7 Chemi-Luminescent ELISA Kit (Human) (OKCD03611)

Catalog No: OKCD03611

Size:96WELLS

Human IGFBP7 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-102%	87-101%	93-101%	94-105%
  EDTA Plasma (n=5)	84-101%	82-99%	83-101%	80-89%
  Heparin Plasma (n=5)	89-104%	84-98%	82-96%	96-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Insulin Like Growth Factor Binding Protein 7 (IGFBP7) level in the sample or standard.
• ELISA Kit Range: 0.82-600ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-94	88
  EDTA Plasma (n=5)	86-93	90
  Heparin Plasma (n=5)	78-90	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 7 (IGFBP7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 7 (IGFBP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IGFBP7 Microplate: 96 Wells (12 x 8 Well strips)
  • IGFBP7 Lyophilized Standard: 2
  • 100X IGFBP7 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 9.38 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGFBP7
• Gene Full Name: insulin like growth factor binding protein 7
• Alias Symbols: AGM;angiomodulin;FSTL2;IBP-7;IGF-binding protein 7;IGFBP-7;IGFBP-7v;IGFBP-rP1;IGFBPRP1;insulin-like growth factor-binding protein 7;MAC25;MAC25 protein;PGI2-stimulating factor;prostacyclin-stimulating factor;PSF;RAMSVPS;TAF;tumor-derived adhesion factor.
• NCBI Gene Id: 3490
• Protein Name: Insulin-like growth factor-binding protein 7
• Description of Target: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
• Uniprot ID: Q16270
• Protein Accession #: NP_001240764
• Nucleotide Accession #: NM_001253835