IGFBP5 ELISA Kit (Rat) (OKBB00590)

Catalog No: OKBB00590

Size:96 Wells

Rat IGFBP5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's rat IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP5 has been precoated onto 96-well plates. Standards(NS0, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat IGFBP5 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.32	4.14	6.88	1.45	4.24	7.26
  SD	0.055	0.162	0.234	0.096	0.225	0.348
  CV%	4.2	3.9	3.4	6.6	5.3	4.8

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Rat IGFBP5 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Rat IGFBP5 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Rat IGFBP5 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant rat IGFBP5
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L20-E271
• Description: For quantitative detection of rat IGFBP5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: IGFBP5
• Gene Full Name: insulin-like growth factor binding protein 5
• Alias Symbols: IBP 5, IBP-5, IBP5, IBP5_HUMAN, IGF binding protein 5, IGF BP5, IGF-binding protein 5, IGFBP 5, IGFBP-5, IGFBP5, Insulin like growth factor binding protein 5, Insulin-like growth factor-binding protein 5
• NCBI Gene Id: 25285
• Protein Name: Insulin-like growth factor-binding protein 5
• Description of Target: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.
• Uniprot ID: P24594
• Protein Accession #: NP_036949.1
• Nucleotide Accession #: NM_012817.1