Catalog No: OKCD02619
Size:96WELLS
Price: $625.00
SKU
OKCD02619
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Datasheets/ManualsPrintable datasheet for IGFBP4 ELISA Kit (Mouse) (OKCD02619)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)84-98%78-92%82-104%78-102%
EDTA Plasma (n=5)95-103%98-105%97-105%82-103%
Heparin Plasma (n=5)94-104%79-89%86-95%97-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 4 (IGFBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-104101
EDTA Plasma (n=5)98-105102
Heparin Plasma(n=5)89-9793
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IGFBP4 Microplate96 Wells (12 x 8 Well strips)
IGFBP4 Lyophilized Standard2
100X Biotinylated IGFBP4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.065 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 4 (IGFBP4). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolIgfbp4
Gene Full Nameinsulin-like growth factor binding protein 4
Alias SymbolsAI875747;Band 3A;Deb2;differentially expressed in B16F10 2;IBP-4;IGF-binding protein 4;IGFBP;IGFBP-4;insulin-like growth factor-binding protein 4.
NCBI Gene Id16010
Protein NameInsulin-like growth factor-binding protein 4
Description of TargetIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Uniprot IDP47879
Protein Accession #NP_034647.1
Nucleotide Accession #NM_010517.3
Protein Size (# AA)254
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