IGFBP4 ELISA Kit (Human) (OKBB00713)

Catalog No: OKBB00713

Size:96 wells

IGFBP4 ELISA Kit (Human) (OKBB00713) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human IGFBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP4 has been precoated onto 96-well plates. Standards (NS0, D22-E258) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IGFBP4 amount of sample captured in plate.
• ELISA Kit Range: 500 pg/ml - 32,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	634	3283	15689	630	3596	16628
  Standard deviation	29.16	164.15	957.02	40.32	222.95	1330.24
  CV(%)	4.6	5	6.1	6.4	6.2	8

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human IGFBP4 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human IGFBP4 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human IGFBP4 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	634	669	599	583	621	33.16	5.3%
  Sample 2	3283	3066	3571	3160	3270	190.05	5.8%
  Sample 3	15689	15202	16042	14388	15330	620.39	4%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	500	1000	2000	4000	8000	16000	32000
  O.D.	0.031	0.169	0.200	0.280	0.401	0.660	1.140	1.880

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <20 pg/ml
• Specificity: Natural and recombinant human IGFBP4
• Immunogen: Expression system for standard: NS0; Immunogen sequence: D22-E258
• Description: For quantitative detection of human IGFBP4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: IGFBP4
• Gene Full Name: insulin-like growth factor binding protein 4
• Alias Symbols: AI875747, BP 4, BP4, Deb2, HT29 IGFBP, IBP 4, IBP-4, IBP4, IBP4_HUMAN, IGF binding protein 4, IGF-binding protein 4, IGF-BP4, IGFBP 4, IGFBP-4, IGFBP4, Insulin Like Growth Factor Binding Protein 4, Insulin-like growth factor-binding protein 4
• NCBI Gene Id: 3487
• Protein Name: Insulin-like growth factor-binding protein 4
• Description of Target: Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.
• Uniprot ID: P22692
• Protein Accession #: NP_001543.2
• Nucleotide Accession #: NM_001552.2