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Catalog No: OKEH03994
Size:96T
Price: $325.00
SKU
OKEH03994
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Igfbp2 ELISA Kit (Mouse) (OKEH03994) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igfbp2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Igfbp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igfbp2 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.8% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Igfbp2 Microplate96 Wells (12 x 8 Well Strips)
Igfbp2 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Igfbp2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.082 ng/mL
SpecificityNatural and recombinant Mouse Insulin-like growth factor-binding protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIgfbp2
Gene Full Nameinsulin-like growth factor binding protein 2
Alias SymbolsAI255832;IBP-2;IGF-binding protein 2;IGFBP;Igfbp-2;insulin-like growth factor-binding protein 2;mIGFBP-2.
NCBI Gene Id16008
Protein NameP47877
Description of TargetInsulin-like growth factor-binding protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/P47877
Protein Accession #NP_032368.2
Nucleotide Accession #NM_008342.3
Protein Size (# AA)305
Molecular Weight33 kDa
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