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Catalog No: OKEH00084
Price: $325.00
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IGFBP2 ELISA Kit (Human) : 96 Wells (OKEH00084)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00084
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IGFBP2 ELISA Kit (Human) (OKEH00084) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP2 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.6% (n = 20)
Mean Inter-assay CV%: < 9.9% (n = 20)
ELISA Kit Component
IGFBP2 Microplate96 Wells (12 x 8 Well strips)
IGFBP2 Lyophilized Standard2
100X Biotinylated IGFBP2 Detector Antibody1 x 20 mL
100X Avidin-HRP Conjugate1 x 60 uL
Sample Diluent1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human Insulin-like growth factor-binding protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIGFBP2
Gene Full Nameinsulin like growth factor binding protein 2
Alias SymbolsIBP2;IGF-binding protein 2;IGF-BP53;insulin-like growth factor binding protein 2, 36kDa;insulin-like growth factor-binding protein 2.
NCBI Gene Id3485
Protein NameP18065
Description of TargetInsulin-like growth factor-binding protein 2
Uniprot ID
Protein Accession #NP_000588
Nucleotide Accession #NM_000597
Protein Size (# AA)325
Molecular Weight35 kDa
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