IGFBP1 ELISA Kit (Bovine) (OKEH07252)

Catalog No: OKEH07252

Size:96T

Bovine IGFBP1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology IGFBP1 ELISA Kit (Bovine) (OKEH07252) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.7% (n = 20)
  Mean Inter-assay CV%: <=8.7% (n = 20)
• ELISA Kit Component:
  • IGFBP1 Microplate: 96 Wells (12 x 8 Well strips)
  • IGFBP1 Lyophilized Standard: 2
  • 100X Biotinylated IGFBP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
  Subcellular Location: Secreted
  Unigene: Bt.190
  SMR: P24591
  String: 9913.ENSBTAP00000055280
  KEGG: bta:282259
  Pfam: PF00219
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.078ng/mL
• Specificity: Natural and recombinant Bovine Insulin-like growth factor-binding protein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGFBP1
• Gene Full Name: insulin like growth factor binding protein 1
• Alias Symbols: bIGFBP-1;IBP-1;IGF-binding protein 1;IGFBP-1;insulin-like growth factor-binding protein 1.
• NCBI Gene Id: 282259
• Protein Name: P24591
• Description of Target: Insulin-like growth factor-binding protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/P24591
• Protein Accession #: NP_776979.2
• Nucleotide Accession #: NM_174554.3