Catalog No: OOPA00044 (Formerly GWB-BSP045)
Size:5UG
Price: $75.00
SKU
OOPA00044
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OOPA00044 |
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Predicted Species Reactivity | Human |
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Product Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
Host | HEK293 cells. |
Additional Information | Solubility: It is recommended to Reconstitution: Reconstitute the lyophilized IGFBP4 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Product Introduction: IGFBP-4 Human Recombinant amino acids Asp22- Glu258, produced in HEK293 cells and fused with a ployhistide tag at the C-terminus. IGFBP-4 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IGFBP-4 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Formulation | IGFBP 4 was lyophilized after extensive dialysis against PBS. |
Purification | Greater than 95.0% as determined by SDS-PAGE. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Biological Activity | The ED50 range is 0.01-0.09ug/ml as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells. |
Peptide Sequence | DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE + His Tag. |
Gene Symbol | IGFBP 4 |
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Alias Symbols | Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4, BP-4, HT29-IGFBP. |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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