IGFALS ELISA Kit (Mouse) (OKCA00900)

Catalog No: OKCA00900

Size:96 Wells

Mouse IGFALS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  1:100	96	93-99
  1:200	84	80-87
  1:400	91	87-94
  1:800	107	104-110

• ELISA Kit Principle: Aviva Systems Biology Igfals ELISA Kit (Mouse) (OKCA00900) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Igfals has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Igfals is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Igfals captured in well.
• ELISA Kit Range: 3.12 ng/mL-200 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94	90-98
  EDTA Plasma (n=4)	85	81-89

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Igfals were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Igfals were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Igfals Microplate: 96 Wells (12 x 8 Well strips)
  • Igfals Lyophilized Standard: 2
  • 100X Biotinylated Igfals Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.78 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGFALS
• Gene Full Name: Insulin-like growth factor binding protein, acid labile subunit
• Alias Symbols: A, ALS, Albs
• NCBI Gene Id: 16005
• Protein Name: Insulin-like growth factor-binding protein complex acid labile subunit
• Description of Target: May have an important role in regulating the access of circulating IGFs to the tissues.
• Uniprot ID: P70389
• Protein Accession #: NP_032366.2
• Nucleotide Accession #: NM_008340.3