IGFALS Antibody - middle region (OAAB04527)

Catalog No: OAAB04527

Size:400ul

Western blot analysis of IGFALS Antibody (Center) in mouse spleen tissue, Jurkat cell line and mouse liver tissue lysates (35ug/lane). IGFALS (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with IGFALS Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

IGFALS Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC-P
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This IGFALS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-335 amino acids from the Central region of human IGFALS.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: IGFALS Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: IGFALS
• Alias Symbols: ALS, ACLSD
• NCBI Gene Id: 3483
• Protein Name: Insulin-like growth factor-binding protein complex acid labile subunit
• Description of Target: IGFALS is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this protein are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty.
• Uniprot ID: P35858
• Protein Accession #: NP_001139478.1
• Molecular Weight: 66035 Da