IGF2BP2 ELISA Kit (Human) (OKCA01313)

Catalog No: OKCA01313

Size:96 Wells

Human IGF2BP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	85	80-90
  1:2	96	92-100
  1:4	90	85-94
  1:8	104	100-108

• ELISA Kit Principle: Aviva Systems Biology IGF2BP2 ELISA Kit (Human) (OKCA01313) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGF2BP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGF2BP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IGF2BP2 captured in the well.
• ELISA Kit Range: 125 - 8,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	89	84-93
  EDTA Plasma (n=4)	95	90-100

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01313 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01313 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • IGF2BP2 Microplate: 96 Wells (12 x 8 Well strips)
  • IGF2BP2 Lyophilized Standard: 2 x 8 ng
  • 100X Biotinylated IGF2BP2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 31.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: IGF2BP2
• Gene Full Name: insulin like growth factor 2 mRNA binding protein 2
• Alias Symbols: IMP2, IMP-2, VICKZ2
• NCBI Gene Id: 10644
• Protein Name: Insulin-like growth factor 2 mRNA-binding protein 2
• Description of Target: RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.
• Uniprot ID: Q9Y6M1
• Protein Accession #: NP_001007226.1
• Nucleotide Accession #: NM_001007225.2