IGF2 Antibody - middle region (Phospho-R54) (OAAB07463)

Catalog No: OAAB07463

Size:200ul

IGF2 Antibody (Center R54) (Cat. #OAAB07463) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).

IGF2 Antibody (Center R54) (Cat. #OAAB07463) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).

IGF2 Antibody (Center R54) (Cat. #OAAB07463)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry. Clinical relevance has not been evaluated.

IGF2 Antibody (Center R54) (Cat. #OAAB07463) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Function: The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable). Acts as a ligand for integrin which is required for IGF2 signaling.
  Cellular Location: Secreted.
  Tissue Location: Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye and pancreas.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.45 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: FC: 1:25
  WB: 1:2000
  IHC-P: 1:50~100
  WB: 1:1000
• Publications:

  Modulation of IGF2 Expression in the Murine Thymus and Thymic Epithelial Cells Following Coxsackievirus-B4 Infection. Microorganisms. 9 (2021) 33672010

• Description:
  View product citations for IGF2 Antibody - middle region (Phospho-R54) (OAAB07463) on CiteAb

Target Info

• Gene Symbol: IGF2
• Alias Symbols: GRDF, SRS3, IGF-II, PP9974, C11orf43
• NCBI Gene Id: 3481
• Protein Name: Insulin-like growth factor II
• Description of Target: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
  
• Uniprot ID: P01344
• Protein Accession #: NP_000603.1
• Molecular Weight: 20 kDa