IGF1R ELISA Kit (Bovine) (OKEH07796)

Catalog No: OKEH07796

Size:96T

Bovine IGF1R ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology IGF1R ELISA Kit (Bovine) (OKEH07796) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGF1R has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGF1R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGF1R captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.7% (n = 20)
  Mean Inter-assay CV%: <=5.9% (n = 20)
• ELISA Kit Component:
  • IGF1R Microplate: 96 Wells (12 x 8 Well strips)
  • IGF1R Lyophilized Standard: 2
  • 100X Biotinylated IGF1R Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R (By similarity). When present in a hybrid receptor with INSR, binds IGF1.
  Subcellular Location: Cell membrane Single-pass type I membrane protein
  Unigene: Bt.105919
  SMR: Q05688
  String: 9913.ENSBTAP00000028690
  KEGG: bta:281848
  Pfam: PF00041
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 32pg/mL
• Specificity: Natural and recombinant Bovine Insulin-like growth factor 1 receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGF1R
• Gene Full Name: insulin like growth factor 1 receptor
• Alias Symbols: IGF-I receptor;IGFR1;insulin-like growth factor 1 receptor;insulin-like growth factor I receptor;insulin-like growth factor receptor type 1.
• NCBI Gene Id: 281848
• Protein Name: Q05688
• Description of Target: Insulin-like growth factor 1 receptor
• Uniprot ID: https://www.uniprot.org/uniprot/Q05688
• Protein Accession #: NP_001231541.1
• Nucleotide Accession #: NM_001244612.1