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Catalog No: OPPA00481 (Formerly GWB-75633C)
Size:100UG
Price: $75.00
SKU
OPPA00481
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IGF1 Protein (OPPA00481)

Datasheets/ManualsPrintable datasheet for OPPA00481
Product Info
Predicted Species ReactivityHuman
Product FormatThe protein was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
HostE. Coli
Additional InformationSolubility: It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
::Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1x1,000,000 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml.
::Protein Content: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.
Protein Content: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.
::Product Introduction: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).
Product Description: Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton. IGF-I is purified by proprietary chromatographic techniques.
Reconstitution and StorageLyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IGF1 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
PurityGreater than 95.0% as determined by
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.
Peptide SequenceThe sequence of the first five N - terminal amino acids was determined and was found to be Gly - Pro - Glu - Thr - Leu. N - terminal methionine has been completely removed enzymatically.
Gene SymbolIGF1
Alias SymbolsIGF, MGF, IGFI, IGF-I
NCBI Gene Id3479
Protein NameInsulin-like growth factor I
Description of TargetRecombinant Human Insulin Like Growth Factor-1
Uniprot IDP01343
Protein Accession #NP_000609.1
Protein Size (# AA)Recombinant
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