IgE ELISA Kit (Human) (OKIA00069)

Catalog No: OKIA00069 (Formerly GWB-2E2ECD)

Size:96W

Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.

Product Info

• Predicted Species Reactivity: Human
• Product Format: In Solution
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 80 Minutes
• ELISA Kit Principle: Aviva Systems Biology IgE ELISA Kit (Human) (OKIA00069) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Immunoglobulin heavy constant epsilon has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Immunoglobulin heavy constant epsilon - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Immunoglobulin heavy constant epsilon captured in the well.
• ELISA Kit Range: 3.125 ng/ml - 200 ng/ml
• ELISA Kit Component:
  • Anti-Human IgE Microplate: 96 Wells (12 x 8 Well Strips)
  • 20X Diluent Concentrate: 50 mL
  • 20X Wash Solution: 50 mL
  • 100X Enzyme-Antibody Conjugate: 150 uL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
  • Human IgE Calibrator: 1
• Additional Information: Intended Use: The total Human IgE test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in human biological samples.
  Reagents:
  1. DILUENT CONCENTRATE (Running Buffer)
  One bottle containing 50 ml of a 5X concentrated diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 ul of affinity purified anti-Human IgE antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid.
  WARNING: Avoid contact with skin.
  6. ANTI-HUMAN IgE ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human IgE.
  7. HUMAN IgE CALIBRATOR
  One vial containing of Human IgE calibrator.
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.912 ng/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Alias Symbols: IGHE, Ig epsilon chain C region, IgE
• Protein Name: Immunoglobulin heavy constant epsilon
• Description of Target: Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
• Uniprot ID: P01854