IgA ELISA Kit (Human) (OKIA00209)

Catalog No: OKIA00209

Size:1 x 96 well plate

Representative Human IgA Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Human
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 70 Minutes
• ELISA Kit Principle: The Aviva Systems Biology IgA ELISA Kit (Human) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Immunoglobulin A (whole molecule) in Plasma or Serum. This assay employs an antibody specific for Human IgA coated on a 96-well plate. Standards and samples are pipetted into the wells and Immunoglobulin A (whole molecule) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-IgA antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 12.50 - 800 ng/mL
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Human IgA Microplate: 96 Wells (12 x 8 Well Strips)
  • Human IgA Calibrator: 1
  • 100X IgA-HRP Conjugate: 150 uL
  • 5X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA/Immunoglobulin A in Human biological samples.
  Sample Volume: 5 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 2 ng/mL

Target Info

• Alias Symbols: IgA;IGHA1;IGHA2;
• Protein Name: Immunoglobulin A (whole molecule)
• Description of Target: Immunoglobulin A (IgA, also referred to as sIgA in its secretory form) is an antibody that plays a role in the immune function of mucous membranes. The amount of IgA produced in association with mucosal membranes is greater than all other types of antibody combined.