Catalog No: OKEH00516
Size:96 Wells
Price: $325.00
SKU
OKEH00516
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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IgA ELISA Kit (Human) : 96 Wells (OKEH00516)

We suggest the following suitable replacements: OKGD00009
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00516
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IgA ELISA Kit (Human) (OKEH00516) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bovine IgA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Bovine IgA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bovine IgA captured in well.
ELISA Kit Range0.312 - 20ug/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 9.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-IgA Microplate96 Wells (12 x 8 Well strips)
IgA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated IgA1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078ug/mL
SpecificityNatural and recombinant Human Immunoglobulin A
Publications

Macronutrient, immunoglobulin a and total antioxidant capacity profiles of human milk from 1 to 24 months: a cross-sectional study in Thailand. Int Breastfeed J. 15, 90 (2020). 33126900

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIGA
Alias SymbolsImmunoglobulin A
Description of TargetDecreased or absent IgA, termed selective IgA deficiency, can be a clinically significant immunodeficiency. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. More IgA is produced in mucosal linings than all other types of antibody combined; between three and five grams are secreted into the intestinal lumen each day. This accumulates to 75% of the total immunoglobulin produced in the entire body.
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