IgA ELISA Kit (Human) : 96 Wells (OKEH00516)

Catalog No: OKEH00516

Size:96T

We suggest the following suitable replacements: OKGD00009

Bovine IgA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IgA ELISA Kit (Human) (OKEH00516) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bovine IgA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Bovine IgA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bovine IgA captured in well.
• ELISA Kit Range: 62.5-4000pg/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.6% (n = 20)
  Mean Inter-assay CV%: < 9.1% (n = 20)
• ELISA Kit Component:
  • Anti-IgA Microplate: 96 Wells (12 x 8 Well strips)
  • IgA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated IgA: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078ug/mL
• Specificity: Natural and recombinant Human Immunoglobulin A
• Publications:

  Macronutrient, immunoglobulin a and total antioxidant capacity profiles of human milk from 1 to 24 months: a cross-sectional study in Thailand. Int Breastfeed J. 15, 90 (2020). 33126900

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for IgA ELISA Kit (Human) : 96 Wells (OKEH00516) on CiteAb

Target Info

• Gene Symbol: IGA
• Alias Symbols: Immunoglobulin A
• Description of Target: Decreased or absent IgA, termed selective IgA deficiency, can be a clinically significant immunodeficiency. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. More IgA is produced in mucosal linings than all other types of antibody combined; between three and five grams are secreted into the intestinal lumen each day. This accumulates to 75% of the total immunoglobulin produced in the entire body.