IgA ELISA Kit (Feline) (OKIA00194)

Catalog No: OKIA00194

Size:1 x 96 well plate

Representative Feline IgA Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Feline
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 100 Minutes
• ELISA Kit Principle: The Aviva Systems Biology IgA ELISA Kit (Feline) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline Immunoglobulin A (whole molecule) in Plasma or Serum. This assay employs an antibody specific for Feline IgA coated on a 96-well plate. Standards and samples are pipetted into the wells and Immunoglobulin A (whole molecule) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-IgA antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 12.50 - 400 ng/mL
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Feline IgA Microplate: 96 Wells (12 x 8 Well Strips)
  • Feline IgA Calibrator: 1
  • 100X IgA-HRP Conjugate: 150 uL
  • 5X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA/Immunoglobulin A in Feline biological samples.
  Sample Volume: 5 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 4.5 ng/mL

Target Info

• Alias Symbols: IgA;IGHA;
• Protein Name: Immunoglobulin A (whole molecule)
• Description of Target: Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268).