IFNG ELISA Kit (Sheep) (OKCA01021)

Catalog No: OKCA01021

Size:96 Wells

Product Info

• Predicted Species Reactivity: Sheep
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	98	95-102
  1:2	87	84-90
  1:4	85	82-89
  1:8	93	90-96

• ELISA Kit Principle: Aviva Systems Biology IFNG ELISA Kit (Sheep) (OKCA01021) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for IFNG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFNG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IFNG captured in well.
• ELISA Kit Range: 5 pg/mL-200 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92	87-95
  EDTA Plasma (n=4)	86	83-89

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level IFNG were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level IFNG were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <0.1
  Inter-Assay CV: <0.15
• ELISA Kit Component:
  • IFNG Microplate: 96 Wells (12 x 8 Well strips)
  • IFNG Lyophilized Standard: 2
  • 100X Biotinylated IFNG Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 2.5 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IFNG
• Gene Full Name: Interferon gamma
• Alias Symbols: IFN-gamma
• NCBI Gene Id: 443396
• Protein Name: Interferon gamma
• Description of Target: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
• Uniprot ID: P17773
• Protein Accession #: NP_001009803.1
• Nucleotide Accession #: NM_001009803.1