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Catalog No: OKEH00078
Size:96T
Price: $500.00
SKU
OKEH00078
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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IFNG ELISA Kit (Mouse) : 96 Wells (OKEH00078)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00078
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IFNG ELISA Kit (Mouse) (OKEH00078) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFNG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFNG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFNG captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
IFNG Microplate96 Wells (12 x 8 Well strips)
IFNG Lyophilized Standard2
100X Biotinylated IFNG Detector Antibody1 x 20 mL
100X Avidin-HRP Conjugate1 x 60 uL
Sample Diluent1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.96 pg/mL
SpecificityNatural and recombinant Mouse Interferon gamma
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIfng
Gene Full Nameinterferon gamma
Alias Symbolsgamma interferon;If;If2f;Ifg;IFN-g;IFN-gamma;interferon 2f;interferon gamma.
NCBI Gene Id15978
Protein NameP01580
Description of TargetInterferon gamma
Uniprot IDhttps://www.uniprot.org/uniprot/P01580
Protein Accession #NP_032363
Nucleotide Accession #NM_008337
Protein Size (# AA)155
Molecular Weight18 kDa
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