Size:96 Wells
$370.00
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SKU
OKAA00006-96W
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IFNG ELISA Kit (Human) : 96 Wells (OKAA00006-96W)

Catalog#: OKAA00006-96W
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Reproducibility The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component
Components
2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody
Additional Information Sample Type: cell culture supernates, serum, and plasma
:: Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IFN-gamma (8x12)
2. 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
Sample Type cell culture, supernates, serum, and plasma
Sensitivity 7 pg/ml
Predicted Homology Based on Immunogen Sequence Human
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay recognizes both natural and recombinant human GM-CSF. The factors listed below were prepared at 10ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, M-CSF, FGF-4, FGF-5, FGF-6
Recombinant mouse: GM-CSF, IL-1alpha, IL-1beta
Recombinant porcine: PDGF, TGF-beta1
Assay Info Assay Method: Quantitative Sandwich ELISA
Gene Symbol IFNG
Official Gene Full Name interferon, gamma
Alias Symbols IFG, IFI, IFN-gamma, Immune interferon, Interferon gamma
NCBI Gene Id 3458
Protein Name Interferon gamma
Description of Target This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
Swissprot Id P01579
Protein Accession # NP_000610
Nucleotide Accession # NM_000619
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express IFNG.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express IFNG.
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