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Catalog No: OKAA00006-96W
Size:96 Wells
Price: $525.00
SKU
OKAA00006-96W
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit ReproducibilityThe coefficient of variation of both intra-assay and inter-assay were less than 10%.
ELISA Kit Component
Components
2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody
Additional InformationSample Type: cell culture supernates, serum, and plasma
::Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IFN-gamma (8x12)
2. 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
Sample Typecell culture, supernates, serum, and plasma
Sensitivity7 pg/ml
Predicted Homology Based on Immunogen SequenceHuman
SpecificityThis assay recognizes both natural and recombinant human GM-CSF. The factors listed below were prepared at 10ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, M-CSF, FGF-4, FGF-5, FGF-6
Recombinant mouse: GM-CSF, IL-1alpha, IL-1beta
Recombinant porcine: PDGF, TGF-beta1
Assay InfoAssay Method: Quantitative Sandwich ELISA
Gene SymbolIFNG
Gene Full Nameinterferon, gamma
Alias SymbolsIFG, IFI, IMD69
NCBI Gene Id3458
Protein NameInterferon gamma
Description of TargetThis gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
Uniprot IDP01579
Protein Accession #NP_000610
Nucleotide Accession #NM_000619
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