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IFNG ELISA Kit (Human) : 96 Wells (OKAA00006-96W)

  • Catalog#: OKAA00006-96W
  • 1 week
96 Wells
$370.00
In Stock
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Predicted Species Reactivity:
Human
Application:
ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IFN-gamma (8x12)
2. 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
Gene Symbol:
IFNG
Official Gene Full Name:
interferon, gamma
Alias Symbols:
IFG, IFI, IFN-gamma, Immune interferon, Interferon gamma
NCBI Gene Id:
3458
Protein Name:
Interferon gamma
Description of Target:
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
Swissprot Id:
P01579
Protein Accession #:
NP_000610
Nucleotide Accession #:
NM_000619
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
7 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IFNG.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IFNG.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay recognizes both natural and recombinant human GM-CSF. The factors listed below were prepared at 10ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, M-CSF, FGF-4, FGF-5, FGF-6
Recombinant mouse: GM-CSF, IL-1alpha, IL-1beta
Recombinant porcine: PDGF, TGF-beta1
Assay Info:
Assay Method: Quantitative Sandwich ELISA

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