Catalog No: OKAA00006-96W
Size:96 Wells
Price: $525.00
SKU
OKAA00006-96W
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | ||||||||||||
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Application | ELISA | ||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||
ELISA Kit Reproducibility | The coefficient of variation of both intra-assay and inter-assay were less than 10%. | ||||||||||||
ELISA Kit Component |
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Additional Information | Sample Type: cell culture supernates, serum, and plasma | ||||||||||||
:: | Reagents: REAGENTS 1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IFN-gamma (8x12) 2. 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution 3. 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma polyclonal antibody 4. 2 vials Streptavidin-HRP solution, 5. 1 bottle Standard /sample Diluent 6. 1 bottle Biotin-Conjugate antibody Diluent 7. 1 bottle Streptavidin-HRP Diluent 8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 9. 1 vial Substrate Solution | ||||||||||||
Sample Type | cell culture, supernates, serum, and plasma | ||||||||||||
Sensitivity | 7 pg/ml | ||||||||||||
Predicted Homology Based on Immunogen Sequence | Human | ||||||||||||
Specificity | This assay recognizes both natural and recombinant human GM-CSF. The factors listed below were prepared at 10ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed. Recombinant human: G-CSF, M-CSF, FGF-4, FGF-5, FGF-6 Recombinant mouse: GM-CSF, IL-1alpha, IL-1beta Recombinant porcine: PDGF, TGF-beta1 | ||||||||||||
Assay Info | Assay Method: Quantitative Sandwich ELISA |
Gene Symbol | IFNG |
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Gene Full Name | interferon, gamma |
Alias Symbols | IFG, IFI, IMD69 |
NCBI Gene Id | 3458 |
Protein Name | Interferon gamma |
Description of Target | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. |
Uniprot ID | P01579 |
Protein Accession # | NP_000610 |
Nucleotide Accession # | NM_000619 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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