Catalog No: OKCD03596
Size:96WELLS
Price: $925.00
SKU
OKCD03596
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IFNG Chemi-Luminescent ELISA Kit (Pig) (OKCD03596)

Datasheets/ManualsPrintable datasheet for IFNG Chemi-Luminescent ELISA Kit (Pig) (OKCD03596)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)91-102%83-92%98-105%79-97%
EDTA Plasma (n=5)99-105%85-94%87-104%89-103%
Heparin Plasma (n=5)99-105%94-101%88-97%95-105%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma (IFNg). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Gamma (IFNg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Gamma (IFNg) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)87-9591
EDTA Plasma (n=5)93-10498
Heparin Plasma (n=5)81-10495
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ifng Microplate96 Wells (12 x 8 Well strips)
Ifng Lyophilized Standard2
100X Ifng Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIFNG
Gene Full Nameinterferon gamma
Alias SymbolsIFN-gamma;interferon gamma.
NCBI Gene Id396991
Protein NameInterferon gamma
Description of TargetProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Uniprot IDP17803
Protein Accession #NP_999113
Nucleotide Accession #NM_213948
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