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IFNG Antibody - PE Conjugated (OASA00343)

100 Tests
$759.00
In Stock
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Gene Symbol:
IFNG
NCBI Gene Id:
281237
Protein Name:
Interferon gamma
Swissprot Id:
P07353
Protein Accession #:
NP_776511.1
Replacement Item:
This antibody may replace item sc-101424 from Santa Cruz Biotechnology.
Protein Size (# AA):
166
Molecular Weight:
20 kDa
Host:
Mouse
Clonality:
Monoclonal
Purification:
Affinity chromatography on Protein G tissue culture supernatant.
Application:
FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IFNG.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IFNG.
Predicted Species Reactivity:
Cow, Human, Pig, Dog, Horse, Sheep, Goat, Dolphin, Ferret, Mink, Fin Whale, Rabbit
Predicted Homology Based on Immunogen Sequence:
Mustelid
Product Format:
Lyophilized from PBS with 0.09% sodium azide, 1% BSA, and 5% sucrose.
Reconstitution and Storage:
Reconstitution: Reconstitute with 1 ml distilled water
Storage: Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Datasheets/Manuals:
Printable datasheet for anti-IFNG antibody - OASA00343
Specificity:
Mouse anti Bovine IFNG antibody, clone CC302, recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferitive properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNG is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodes et al. 2000).

Mouse anti bovine G inerferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersen et al. 2002). Clone CC302 has been successfully used for the evaluation of G interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis (Appana et al. 2013) as a detection reagent using an ELISA.
Clone:
CC302
Conjugation:
PE
Isotype:
IgG1
Application Info:
FC Neat 1:10. Membrane permeabilization is required for this application. Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
:::
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Target Reference:
1. Hasvold, H. et al. (2002) In vitro responses to purified protein derivate of caprine T lymphocytes following vaccination with live strains of Mycobacterium avium subsp paratuberculosis. Vet. Immunol. Immunopathol. 90: 79 - 89.
2. Mwang, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen specific CD4+ T cell responses induced by DNA vaccination of outbred animals. J. Immunol. 169: 3837 - 3846.
3. Pedersen, L. G. et al. (2002) Identification of monoclonal antibodies that cross react with cytokines from different animal species. Vet. Immunol. Immunopathol. 88: 111 - 122.
4. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Immunol. 9: 1229 - 1234.
5. Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virus

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