Catalog No: OKEH07350
Size:96T
Price: $800.00
SKU
OKEH07350
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology IFNB1 ELISA Kit (Bovine) (OKEH07350) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFNB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFNB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFNB1 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.2% (n = 20)
Mean Inter-assay CV%: <=8.1% (n = 20)
ELISA Kit Component
ComponentAmount
IFNB1 Microplate96 Wells (12 x 8 Well strips)
IFNB1 Lyophilized Standard2
100X Biotinylated IFNB1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Has antiviral, antibacterial and anticancer activities.
::Subcellular Location: Secreted
::Unigene: Bt.45040
SMR: P01578
String: 9913.ENSBTAP00000055343
::KEGG: bta:281845
Pfam: PF00143
Reconstitution and StorageStore as indicated in product manual.
Sensitivity6.7pg/mL
SpecificityNatural and recombinant Bovine Interferon beta-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIFNB1
Gene Full Nameinterferon, beta 1, fibroblast
Alias SymbolsIFNb;interferon beta;interferon beta-1.
NCBI Gene Id281845
Protein NameP01578
Description of TargetInterferon beta-1
Uniprot IDhttps://www.uniprot.org/uniprot/P01578
Protein Accession #NP_776775.1
Nucleotide Accession #NM_174350.1
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