IFNB High Sensitivity ELISA Kit (Chicken) (OKCD01319)

Catalog No: OKCD01319

Size:96WELLS

IFNB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Chicken|Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-104%	99-105%	81-105%	89-104%
  EDTA Plasma (n=5)	80-98%	94-102%	78-97%	79-92%
  Heparin Plasma (n=5)	78-102%	95-103%	82-93%	80-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interferon Beta (IFNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interferon Beta (IFNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interferon Beta (IFNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Interferon Beta (IFNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-105	99
  EDTA Plasma (n=5)	78-99	86
  Heparin Plasma (n=5)	82-90	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interferon Beta (IFNb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IFNB Microplate: 96 Wells (12 x 8 Well Strips)
  • IFNB Lyophilized Standard: 2
  • 100X Biotinylated IFNB Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.16 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Beta (IFNb). No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IFNW1
• Gene Full Name: interferon omega 1
• Alias Symbols: IFN_B;IFNB;IFN-beta;interferon type B;interferon, beta 1, fibroblast;interferon-beta.
• NCBI Gene Id: 554219
• Protein Name: Interferon type B
• Description of Target: Has antiviral activities.
• Uniprot ID: Q90873
• Protein Accession #: NP_001020007.1
• Nucleotide Accession #: NM_001024836.1