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Catalog No: OKCD08611
Size:96WELLS
Price: $800.00
SKU
OKCD08611
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Datasheets/ManualsPrintable datasheet for IFNAR2 ELISA Kit (Human) (OKCD08611)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha/Beta Receptor 2 (IFNa/bR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha/Beta Receptor 2 (IFNa/bR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha/Beta Receptor 2 (IFNa/bR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Alpha/Beta Receptor 2 (IFNa/bR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha/Beta Receptor 2 (IFNa/bR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha/Beta Receptor 2 (IFNa/bR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
IFNAR2 Microplate96 Wells (12 x 8 Well strips)
IFNAR2 Lyophilized Standard2
100X IFNAR2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, urine and other biological fluids.
Sensitivity< 0.262ng/mL
Gene SymbolIFNAR2
Gene Full Nameinterferon alpha and beta receptor subunit 2
Alias Symbolshuman interferon alpha/beta receptor;IFNABR;IFN-alpha/beta receptor 2;IFNalpha/beta receptor subunit 2;IFN-alpha-REC;IFNARB;IFN-R;IFN-R-2;IMD45;interferon (alpha, beta and omega) receptor 2;interferon alpha binding protein;interferon alpha/beta receptor 2;interferon receptor;interferon-alpha/beta receptor beta chain;type I interferon receptor 2.
NCBI Gene Id3455
Protein NameInterferon alpha/beta receptor 2
Description of TargetAssociates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation (PubMed:26424569). Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1 (PubMed:8181059, PubMed:7665574, PubMed:7759950). Isoform 3 is a potent inhibitor of type I IFN receptor activity (PubMed:7759950).
Uniprot IDP48551
Protein Accession #NP_000865.2
Nucleotide Accession #NM_000874.4
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