IFNAR2 ELISA Kit (Human) (OKCD08611)

Catalog No: OKCD08611

Size:96WELLS

IFNAR2 ELISA Kit (Human) (OKCD08611) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha/Beta Receptor 2 (IFNa/bR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha/Beta Receptor 2 (IFNa/bR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha/Beta Receptor 2 (IFNa/bR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Alpha/Beta Receptor 2 (IFNa/bR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha/Beta Receptor 2 (IFNa/bR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha/Beta Receptor 2 (IFNa/bR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IFNAR2 Microplate: 96 Wells (12 x 8 Well strips)
  • IFNAR2 Lyophilized Standard: 2
  • 100X IFNAR2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine and other biological fluids.
• Sensitivity: < 0.262ng/mL

Target Info

• Gene Symbol: IFNAR2
• Gene Full Name: interferon alpha and beta receptor subunit 2
• Alias Symbols: human interferon alpha/beta receptor;IFNABR;IFN-alpha/beta receptor 2;IFNalpha/beta receptor subunit 2;IFN-alpha-REC;IFNARB;IFN-R;IFN-R-2;IMD45;interferon (alpha, beta and omega) receptor 2;interferon alpha binding protein;interferon alpha/beta receptor 2;interferon receptor;interferon-alpha/beta receptor beta chain;type I interferon receptor 2.
• NCBI Gene Id: 3455
• Protein Name: Interferon alpha/beta receptor 2
• Description of Target: Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation (PubMed:26424569). Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1 (PubMed:8181059, PubMed:7665574, PubMed:7759950). Isoform 3 is a potent inhibitor of type I IFN receptor activity (PubMed:7759950).
• Uniprot ID: P48551
• Protein Accession #: NP_000865.2
• Nucleotide Accession #: NM_000874.4