Catalog No: OKCD03576
Size:96WELLS
Price: $881.00
SKU
OKCD03576
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IFNA7 Chemi-Luminescent ELISA Kit (Dog) (OKCD03576)

Datasheets/ManualsPrintable datasheet for IFNA7 Chemi-Luminescent ELISA Kit (Dog) (OKCD03576)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)78-101%91-98%78-93%94-102%
EDTA Plasma (n=5)78-94%81-104%85-93%80-91%
Heparin Plasma (n=5)81-88%86-94%78-93%91-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha (IFNa) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-10296
EDTA Plasma (n=5)88-9692
Heparin Plasma (n=5)79-9784
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ifna7 Microplate96 Wells (12 x 8 Well strips)
Ifna7 Lyophilized Standard2
100X Ifna7 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLOC100685213|LOC100687928|LOC100688084
Gene Full Nameinterferon alpha-1/2
Alias Symbolsinterferon alpha-1/2;LOW QUALITY PROTEIN: interferon alpha-1/2.
NCBI Gene Id100685213|100687928|100688084
Protein NameInterferon alpha-1/2
Description of TargetProduced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Uniprot IDP81255
Protein Accession #XP_003431605
Nucleotide Accession #XM_003431557
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