Size:96 Wells
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IFNA2 ELISA Kit (Mouse) (OKEH03517)

Catalog#: OKEH03517
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology IFNA2 ELISA Kit (Mouse) (OKEH03517) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ifna2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ifna2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ifna2 captured in well.
Kit Range 7.8 - 500pg/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
Kit Reproducibility Mean Intra-assay CV%: <= 6.1% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
Kit Component
Ifna2 Microplate96 Wells (12 x 8 Well strips)
Ifna2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Ifna2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 3.9 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Interferon alpha-2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol IFNA2
Official Gene Full Name interferon alpha 2
Alias Symbols Ifa2, IFN-alpha-2, Interferon alpha-2
NCBI Gene Id 15965
Protein Name Interferon alpha-2
Description of Target Produced by macrophages, IFN-alpha have antiviral activities.
Swissprot Id P01573
Protein Accession # NP_034633.2
Nucleotide Accession # NM_010503.2
Protein Size (# AA) 190
Molecular Weight 22 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Ifna2.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Ifna2.
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