IFNA2 Chemi-Luminescent ELISA Kit (Human) (OKCD03586)

Catalog No: OKCD03586

Size:96WELLS

Human IFNA2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	86-94%	78-98%	89-103%	82-99%
  EDTA Plasma (n=5)	95-102%	82-91%	78-97%	83-93%
  Heparin Plasma (n=5)	82-96%	92-101%	92-99%	96-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 2 (IFNa2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha 2 (IFNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha 2 (IFNa2) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	91-105	98
  EDTA Plasma (n=5)	98-105	101
  Heparin Plasma (n=5)	83-105	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IFNA2 Microplate: 96 Wells (12 x 8 Well strips)
  • IFNA2 Lyophilized Standard: 2
  • 100X IFNA2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.37 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IFNA2
• Gene Full Name: interferon alpha 2
• Alias Symbols: alpha-2a interferon;IFNA;IFNA2B;IFN-alpha-2;IFN-alphaA;interferon alpha 2a;interferon alpha 2b;interferon alpha A;interferon alpha-2;Interferon alpha-A;leIF A.
• NCBI Gene Id: 3440
• Protein Name: Interferon alpha-2
• Description of Target: Produced by macrophages, IFN-alpha have antiviral activities.
• Uniprot ID: P01563
• Protein Accession #: NP_000596
• Nucleotide Accession #: NM_000605