Size:96 Wells
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IFNA1 ELISA Kit (Rat) (OKEH03012)

Catalog#: OKEH03012
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species ReactivityRat
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology IFNA1 ELISA Kit (Rat) (OKEH03012) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ifna1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ifna1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ifna1 captured in well.
Kit Range31.2 - 2000pg/ml
Kit Component
Anti-Ifna1 Microplate96 Wells (12 x 8 Well strips)
Ifna1 Lyophilized Standard2
100X Biotinylated Ifna1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity15.6 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Rat Interferon alpha-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIFNA1
Official Gene Full Nameinterferon-alpha 1
Alias SymbolsInterferon alpha-1
NCBI Gene Id298210
Protein NameInterferon alpha-1
Description of TargetProduced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Swissprot IdP05011
Protein Accession #NP_001014786.1
Nucleotide Accession #NM_001014786.1
Protein Size (# AA)192
Molecular Weight22 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Ifna1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Ifna1.
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