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Catalog No: OKDB00037
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OKDB00037
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IFN gamma ELISpot Assay Kit (Rat) (OKDB00037)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva's Rat IFN gamma ELISpot Kit is based on standard sandwich ELISA methodology. A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.
ELISA Kit ComponentReagents sufficient for 96 Wells:
1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells)
2. Biotinylated detection antibody
3. Streptavidin-Alkaline Phosphatase conjugate
4. Bovine Serum Albumin
5. Ready to use BCIP/NBT substrate buffer
Reconstitution and Storage2 to 8C, Do not use past expiration date.
Sample TypeCell Suspensions
Assay InfoAssay Methodology: Quantitative Sandwich ELISpot Immunoassay
Gene SymbolIFN
Gene Full Nameinterferon gamma
Alias SymbolsIf2f, IFNG2
NCBI Gene Id25712
Protein Nameinterferon gamma
Description of Targetan immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006]
Uniprot IDP01581
Protein Accession #NP_620235.1
Nucleotide Accession #NM_138880.2
Protein Size (# AA)156
Molecular Weight18 kDa
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