IFN gamma ELISpot Assay Kit (Murine) (OKDB00035)

Catalog No: OKDB00035

Product Info

• Predicted Species Reactivity: Murine
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Principle: Aviva's Murine IFN gamma ELISpot Kit is based on standard sandwich ELISA methodology. Capture antibodies highly specific for the analyte of interest are coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and finally washed before adding the cells to be investigated. Cell suspension and stimulant are added to the coated and blocked microtitre plate and the plate incubated allowing the specific antibodies to bind any analytes produced. Biotinylated detection antibodies are then added which bind to the previously captured analyte. Enzyme conjugated Streptavidin is added binding to the detection antibodies. Any excess unbound analyte and antibodies are removed by careful washing. Colour substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using microscopes.
• ELISA Kit Component: Reagents sufficient for 96 Wells:
  1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells)
  2. Biotinylated detection antibody
  3. Streptavidin-Alkaline Phosphatase conjugate
  4. Bovine Serum Albumin
  5. Ready to use BCIP/NBT substrate buffer
  
• Reconstitution and Storage: 2 to 8C, Do not use past expiration date.
• Sample Type: Cell Suspensions
• Assay Info: Assay Methodology: Quantitative Sandwich ELISpot Immunoassay

Target Info

• Gene Symbol: IFN
• Gene Full Name: interferon gamma
• Alias Symbols: If, Ifg, If2f, IFN-g
• NCBI Gene Id: 15978
• Protein Name: interferon gamma
• Description of Target: This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
• Uniprot ID: P01580
• Protein Accession #: NP_032363.1
• Nucleotide Accession #: NM_008337.4
• Protein Size (# AA): 155
• Molecular Weight: 18 kDa