Catalog No: OPPA00499 (Formerly GWB-E2B24D)
Size:2UG
Price: $75.00
SKU
OPPA00499
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPPA00499 |
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Predicted Species Reactivity | Human |
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Product Format | Lyophilized from 1mg/ml solution containing 50mg Human Albumin & 50mg dextrose. |
Host | E. Coli |
Additional Information | Solubility: It is recommended to reconstitute the lyophilized Interferon beta-1b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Biological Activity: The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32,000,000 IU/mg. |
:: | Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard. |
:: | Product Introduction: Interferon-beta 1b has antiviral, antibacterial and anticancer activities. Product Description: Interferon beta 1b Human Recombinant produced in E.Coli is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton.The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17. IFN beta-1b is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized Interferon-beta 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IFNB 1b should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles. |
Purity | Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and was found to be Ser - Tyr - Asn - Leu - Leu. |
Gene Symbol | IFN b 1b |
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Alias Symbols | IFB, IFF, IFNB, IFN-beta |
NCBI Gene Id | 3456 |
Protein Name | Interferon beta |
Description of Target | Recombinant Human Interferon-beta 1b |
Uniprot ID | P01574 |
Protein Accession # | NP_002167.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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