IFN b 1a Protein (OPPA00501)

Datasheets/Manuals	Printable datasheet for OPPA00501

Product Info
Predicted Species Reactivity	Human
Product Format	Lyophilized from 1mg/10ml solution containing 91 mg Human Albumin, and 620 mg mannitol, and 9 mg Sodium Acetate.
Host	Chinese Hamster Ovarian Cells (CHO)
Reconstitution and Storage	Reconstitution: It is recommended to reconstitute the lyophilized Interferon beta 1a in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Storage: Lyophilized Interferon-beta 1a although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IFN-Beta1a should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles.
Purification	IFN-beta1a is purified by proprietary chromatographic techniques.
Purity	Greater than 99.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity	The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 270 x 10⁶ IU/mg.
Source	Chinese Hamster Ovarian Cells (CHO)

Gene Symbol	IFN b 1a
Alias Symbols	IFB, IFF, IFNB, IFN-beta
NCBI Gene Id	3456
Protein Name	Interferon beta
Description of Target	Recombinant Human Interferon-beta 1a
Uniprot ID	P01574
Protein Accession #	NP_002167.1
Protein Size (# AA)	Recombinant

Protocol:

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Protein interactions

Biological pathways

Name	# of Products
Activation of cysteine-type endopeptidase activity involved in apoptotic process	193
B cell proliferation	243
Blood coagulation	747
Cellular response to exogenous dsRNA	19
Defense response to virus	284
Induction of apoptosis	395
Natural killer cell activation	93
Negative regulation of cell proliferation	554
Negative regulation of T cell differentiation	18
Negative regulation of T-helper 2 cell cytokine production	18
Negative regulation of viral genome replication	72
Negative regulation of viral transcription	12
Negative regulation of virion penetration into host cell	12
Positive regulation of innate immune response	39
Positive regulation of transcription from RNA polymerase II promoter	1210
Regulation of MHC class I biosynthetic process	12
Regulation of type I interferon-mediated signaling pathway	71
Type I interferon-mediated signaling pathway	159

Biological process

Name	# of Products
Activation of cysteine-type endopeptidase activity involved in apoptotic process	100
Adaptive immune response	23
B cell proliferation	22
Blood coagulation	396
Cell surface receptor signaling pathway	179
Cellular response to exogenous dsRNA	7
Cytokine-mediated signaling pathway	229
Defense response to bacterium	74
Defense response to virus	72
Humoral immune response	51
Induction of apoptosis	226
Innate immune response	300
Natural killer cell activation	18
Negative regulation of cell proliferation	375
Negative regulation of T cell differentiation	10
Negative regulation of T-helper 2 cell cytokine production	5
Negative regulation of viral genome replication	24
Negative regulation of viral transcription	4
Negative regulation of virion penetration into host cell	1
Positive regulation of innate immune response	12
Positive regulation of transcription from RNA polymerase II promoter	968
Regulation of MHC class I biosynthetic process	2
Regulation of type I interferon-mediated signaling pathway	17
Response to virus	137
Type I interferon-mediated signaling pathway	46

Cellular components

Name	# of Products
Extracellular region	1573
Extracellular space	884

Protein function

Name	# of Products
Cytokine activity	1104
Interferon-alpha/beta receptor binding	22
Transcription corepressor activity	425