IFN a 2a Protein (OPPA00469)

Catalog No: OPPA00469 (Formerly GWB-5CFCF9)

Size:20UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Lyophilized lyophilized without additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7 x 10⁸ IU/mg.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IFN-a 2a as a Reference Standard.
  
  Product Introduction: IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
  Product Description: Interferon Alpha Human 2a Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton.The Interferon-a 2a gene was obtained from human leukocytes.The IFN-A 2a is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IFN-alpha 2a should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 98.0% as determined by both(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Cys - Asp - Leu - Pro - Gln, conforming to the sequence of native human IFN - a.N - terminal methionine has been completely removed enzymatically.

Target Info

• Gene Symbol: IFN a 2a
• Alias Symbols: IFNA, IFNA2B, leIF A, IFN-alphaA, IFN-alpha-2
• NCBI Gene Id: 3440
• Protein Name: Interferon alpha-2
• Description of Target: Recombinant Human Interferon-alpha 2a
• Uniprot ID: P01563
• Protein Accession #: NP_000596.2
• Protein Size (# AA): Recombinant