Catalog No: OKEH01672
Size:96T
Price: $525.00
SKU
OKEH01672
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IFIH1 ELISA Kit (Human) : 96 Wells (OKEH01672)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IFIH1 ELISA Kit (Human) (OKEH01672) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFIH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFIH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFIH1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
IFIH1 Microplate96 Wells (12 x 8 Well strips)
IFIH1 Lyophilized Standard2
100X Biotinylated IFIH1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Human Interferon-induced helicase C domain-containing protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIFIH1
Gene Full Nameinterferon induced with helicase C domain 1
Alias SymbolsAGS7;CADM-140 autoantigen;clinically amyopathic dermatomyositis autoantigen 140 kDa;DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide;helicard;helicase with 2 CARD domains;Hlcd;IDDM19;interferon-induced helicase C domain-containing protein 1;Interferon-induced with helicase C domain protein 1;MDA5;MDA-5;melanoma differentiation-associated gene 5;melanoma differentiation-associated protein 5;murabutide down-regulated protein;RIG-I-like receptor 2;RLR-2;RNA helicase-DEAD box protein 116;SGMRT1.
NCBI Gene Id64135
Protein NameQ9BYX4
Description of TargetInterferon-induced helicase C domain-containing protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BYX4
Protein Accession #NP_071451.2
Nucleotide Accession #NM_022168.3
Protein Size (# AA)1025
Molecular Weight117 kDa
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