IFI30 ELISA Kit (Human) (OKCD00443)

Catalog No: OKCD00443 (Formerly GWB-KBBWK2)

Size:96WELLS

Human IFI30 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma Inducible Protein 30 (IFI30). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma Inducible Protein 30 (IFI30). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma Inducible Protein 30 (IFI30), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Gamma Inducible Protein 30 (IFI30) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma Inducible Protein 30 (IFI30) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma Inducible Protein 30 (IFI30) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IFI30 Microplate: 96 Wells (12 x 8 Well Strips)
  • IFI30 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated IFI30 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Gamma Inducible Protein 30 (IFI30). No significant cross-reactivity or interference between Interferon Gamma Inducible Protein 30 (IFI30) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IFI30
• Gene Full Name: IFI30 lysosomal thiol reductase
• Alias Symbols: gamma-interferon-inducible lysosomal thiol reductase;gamma-interferon-inducible protein IP-30;GILT;IFI-30;interferon gamma-inducible protein 30 preproprotein;interferon, gamma-inducible protein 30;IP30;IP-30;legumaturain.
• NCBI Gene Id: 10437
• Protein Name: Gamma-interferon-inducible lysosomal thiol reductase
• Description of Target: Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).
• Uniprot ID: P13284
• Protein Accession #: NP_006323
• Nucleotide Accession #: NM_006332.4
• Protein Size (# AA): 250