IFI30 Antibody (OAGA00272)

Catalog No: OAGA00272

Size:100 ul

Sample(30 ug whole cell lysate)
A:Raji
12% SDS PAGE
OAGA00272 diluted at 1:1000

GILT antibody detects IFI30 protein at cytosol on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
GILT antibody (OAGA00272) dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq]
  Positive Controls: Raji
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 14 and 250 of GILT (Uniprot ID#P13284)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: IFI30
• Gene Full Name: Gamma-interferon-inducible lysosomal thiol reductase
• Alias Symbols: GILT, IP30, IP-30, IFI-30
• NCBI Gene Id: 10437
• Protein Name: Gamma-interferon-inducible lysosomal thiol reductase
• Uniprot ID: P13284
• Protein Accession #: NP_006323.2
• Molecular Weight: 28 kDa