IDO1 ELISA Kit (Rat) (OKCD00281)

Catalog No: OKCD00281 (Formerly GWB-KBBHD1)

Size:96WELLS

Rat Ido1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	82-102%	80-90%	81-96%	91-101%
  EDTA plasma(n=5)	92-99%	94-103%	81-103%	83-99%
  heparin plasma(n=5)	84-92%	82-95%	97-105%	95-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Indoleamine-2,3-Dioxygenase (IDO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Indoleamine-2,3-Dioxygenase (IDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Indoleamine-2,3-Dioxygenase (IDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Indoleamine-2,3-Dioxygenase (IDO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	86-95	92
  EDTA plasma(n=5)	97-104	101
  heparin plasma(n=5)	96-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Indoleamine-2,3-Dioxygenase (IDO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indoleamine-2,3-Dioxygenase (IDO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ido1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ido1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Ido1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.065 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Indoleamine-2,3-Dioxygenase (IDO). No significant cross-reactivity or interference between Indoleamine-2,3-Dioxygenase (IDO) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Ido1
• Gene Full Name: indoleamine 2,3-dioxygenase 1
• Alias Symbols: Ido;IDO-1;Indo;indoleamine 2,3-dioxygenase 1;indoleamine 23-dioxygenase;indoleamine-pyrrole 2,3-dioxygenase.
• NCBI Gene Id: 66029
• Protein Name: Indoleamine 2,3-dioxygenase 1
• Description of Target: Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection.
• Uniprot ID: Q9ERD9
• Protein Accession #: NP_076463
• Nucleotide Accession #: NM_023973.1
• Protein Size (# AA): 407