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Catalog No: OCOA01550
Size:20UG
Price: $179.00
SKU
OCOA01550
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IDH3A Protein Lysate (OCOA01550)

Datasheets/ManualsPrintable datasheet for IDH3A Protein Lysate (OCOA01550)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionIDH3A HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolIDH3A
Gene Full Nameisocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha
Alias SymbolsH-IDH alpha;isocitrate dehydrogenase (NAD(+)) 3 alpha;isocitrate dehydrogenase (NAD+) alpha chain;isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial;isocitrate dehydrogenase 3 (NAD(+)) alpha;isocitrate dehydrogenase 3 (NAD+) alpha;isocitric dehydrogenase subunit alpha;NAD(+)-specific ICDH subunit alpha;NAD(H)-specific isocitrate dehydrogenase alpha subunit;NAD+-specific ICDH;RP90.
NCBI Gene Id3419
Protein NameNM_005530, NP_005521, P50213
Description of TargetHomo sapiens isocitrate dehydrogenase 3 (NAD(+)) alpha (IDH3A), mRNA, Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P50213
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005521
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005530
Molecular Weight39.6 kDa
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