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IDH3A antibody - N-terminal region (ARP42237_T100)

100 ul
$249.00
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Conjugation Options

ARP42237_T100-FITC Conjugated

ARP42237_T100-HRP Conjugated

ARP42237_T100-Biotin Conjugated

Gene Symbol:
IDH3A
NCBI Gene Id:
3419
Official Gene Full Name:
Isocitrate dehydrogenase 3 (NAD+) alpha
Protein Name:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
Swissprot Id:
P50213
Protein Accession #:
NP_005521
Nucleotide Accession #:
NM_005530
Replacement Item:
This antibody may replace item sc-366994 from Santa Cruz Biotechnology.
Description of Target:
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. IDH3A is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Protein Size (# AA):
366
Molecular Weight:
40kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Protein A purified
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IDH3A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IDH3A.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human IDH3A
Tested Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%
Complete computational species homology data:
Anti-IDH3A (ARP42237_T100)
Peptide Sequence:
Synthetic peptide located within the following region: MKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLK
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
HUWE1; SUMO2; STAU1; UBC; MDM2; ADRB2; gag-pol; RAB4A; MBNL1; S100A16; SUCLA2; UQCRFS1P1; SUMO4; EBNA-LP; MYC; TERF2; TERF1; PSMD4; DDA1; DMWD; IDH3B; IDH3G;
Blocking Peptide:
For anti-IDH3A (ARP42237_T100) antibody is Catalog # AAP42237 (Previous Catalog # AAPP24660)
Datasheets/Manuals:
Printable datasheet for anti-IDH3A (ARP42237_T100) antibody
Sample Type Confirmation:

IDH3A is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Subunit:
alpha, mitochondrial
Target Reference:
Soundar,S., (2003) J. Biol. Chem. 278 (52), 52146-52153
Publications:

Lin, C.-C. et al. Loss of the respiratory enzyme citrate synthase directly links the Warburg effect to tumor malignancy. Sci. Rep. 2, 785 (2012). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 23139858

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