IDH2 Antibody - C-terminal region (OABB02085)

Catalog No: OABB02085

Size:100UG

Anti-IDH2 Picoband antibody, OABB02085, Western blotting
All lanes: Anti IDH2 (OABB02085) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: NIH3T3 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD

Anti-IDH2 Picoband antibody, OABB02085, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Anti-IDH2 Picoband antibody, OABB02085, IHC(P)
IHC(P): Mouse Intestine Tissue

Anti-IDH2 Picoband antibody, OABB02085, IHC(P)
IHC(P): Rat Intestine Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Frozen|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Peptide Sequence: Synthetic peptide located within the following region: KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml
  Immunocytochemistry : 0.5-1 ug/ml
  Immunocytochemistry/Immunofluorescence: 2 ug/ml
  Flow Cytometry: 1-3 ug/1x10^6 cells
• Reference: 1. "Entrez Gene: IDH2 isocitrate dehydrogenase 2 (NADP+), mitochondrial".
  2. Xu X, Zhao J, Xu Z, Peng B, Huang Q, Arnold E, Ding J (Aug 2004). "Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity". The Journal of Biological Chemistry 279 (32): 33946–57.
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Isocitrate dehydrogenase [NADP], mitochondrial(IDH2) detection. Tested with WB, IHC-P, IHC-F, ICC, ICC/IF, FCM in Human; Mouse;Rat.

Target Info

• Gene Symbol: IDH2
• Gene Full Name: isocitrate dehydrogenase (NADP(+)) 2
• Alias Symbols: D2HGA2;ICD-M;IDH;IDHM;IDP;IDPM;isocitrate dehydrogenase (NADP(+)) 2, mitochondrial;isocitrate dehydrogenase [NADP], mitochondrial;isocitrate dehydrogenase 2 (NADP+), mitochondrial;mNADP-IDH;NADP(+)-specific ICDH;oxalosuccinate decarboxylase.
• NCBI Gene Id: 3418
• Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial
• Description of Target: Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
• Uniprot ID: P48735
• Molecular Weight: 50909 MW