IDH1 Antibody - C-terminal region (ARP54787_P050)

Catalog No: ARP54787_P050

Host: Rat
Target Name: IDH1
Sample Tissue: Rat Brain
Antibody Dilution: 1ug/ml

IDH1 antibody - C-terminal region (ARP54787_P050) validated by WB using HEPG2 lysate + Drosophila extract at 1:1000.IDH1 is supported by BioGPS gene expression data to be expressed in HepG2

Rabbit Anti-IDH1 Antibody
Catalog Number: ARP54787
Paraffin Embedded Tissue: Human Testis
Antibody Concentration: 5 ug/ml

Rabbit Anti-IDH1 Antibody
Catalog Number: ARP54787
Paraffin Embedded Tissue: Human Testis
Antibody Concentration: 5 ug/ml

Sample Type:
Human glioma cells
Primary Antibody Dilution:
1:200
Secondary Antibody:
Anti-rabbit-GFP
Secondary Antibody Dilution:
1:5000
Color/Signal Descriptions:
1. Red: Nucleus 2. Green: IDH 3. Merge
Gene Name:
IDH1
Submitted by:
ELIAS EL HABR, PhD, Post-Doctoral fellow, INSERM U894 Glial Plasticity Team, Psychiatry& Neurosciences Center, St Anne Hospital, 2 Ter, Rue d'Alesia, 75014 Paris

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Yeast
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Additional Information: IHC Information: Human Testis (formalin-fixed, paraffin-embedded) stained with IDH1 antibody ARP54787_P050 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  IHC Information: Human Testis (formalin-fixed, paraffin-embedded) stained with IDH1 antibody ARP54787_P050 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human IDH1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Yeast: 93%
• Peptide Sequence: Synthetic peptide located within the following region: MMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRG
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-IDH1 (ARP54787_P050) antibody is Catalog # AAP54787 (Previous Catalog # AAPP31582)
• Sample Type Confirmation:

  IDH1 is supported by BioGPS gene expression data to be expressed in HepG2

• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

Target Info

• Reference: Kullberg,M., (2006) Mol. Biol. Evol. 23 (8), 1493-1503
• Gene Symbol: IDH1
• Gene Full Name: Isocitrate dehydrogenase 1 (NADP+), soluble
• Alias Symbols: IDH, IDP, IDCD, IDPC, PICD, HEL-216, HEL-S-26
• NCBI Gene Id: 3417
• Protein Name: Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial
• Description of Target: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+).The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: O75874
• Protein Accession #: NP_005887
• Nucleotide Accession #: NM_005896
• Protein Size (# AA): 414
• Molecular Weight: 47 kDa
• Protein Interactions: SUMO2; UBC; GLRX3; UBE2H; THOP1; TALDO1; RCN1; RBBP7; RANBP1; PTMS; OXCT1; NME1; HSPE1; GOT1; FDPS; DCK; ANXA6; ADSS; NME1-NME2; EFHD2; C12orf10; SBDS; CHMP4A; CBX3; SUGT1; PEX5; AK2; AK1; MDH2; ASF1B; CDK2; SLC2A4; ELAVL1; SUMO4; ZHX1;